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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Nuclear Pore Complex, Panspecific

Anti -Nuclear Pore Complex, Panspecific

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant IF
031416 was one of a series of clones which strongly and specifically labelled the nuclear pore complex. When this antibody was tested on cells from other species, including rat, mouse and human cells, it has invariably strongly stained nuclear pore complexes, so it appears to be an excellent and panspecific marker for these important structures. 031416 does not work well on Western Blots so we are currently unsure of the exact identity of the protein to which it binds.
Catalog #031416
ApplicationsSuitable for use in Immunofluorescence. Other applications not tested.
Recommended DilutionImmunofluorescence: For yeast cells, try diluted 1:100-1:500. For immunofluorescence on mammalian cells try at 1:50-1:100.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No13B850
HostMouse
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium azide.
PuritySterile-filtered cell culture fluid from an Integra CL-350 biochamber
ImmunogenRaised by injecting mice with yeast nuclear preparations and screening the resullting hybridomas by immunofluorescence on yeast cells.
SpecificityRecognizes Nuclear Pore Complex antigen in human HeLa cells. Species Crossreactivity: Mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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