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You are here:Home » Antibodies » Abs to Splicing Factors » Anti -Splicing Factor 3B Subunit 4 (SF3B4, SF3b50, Pre-mRNA Splicing Factor SF3b 49kD Subunit, Spliceosome-associated Protein 49, SAP 49, SAP49)

Anti -Splicing Factor 3B Subunit 4 (SF3B4, SF3b50, Pre-mRNA Splicing Factor SF3b 49kD
Subunit, Spliceosome-associated Protein 49, SAP 49, SAP49)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IF
Splicing factor SF3B4, also known as SAP49, is a ubiquitously expressed splicing factor found in the nuclei of eukaryotic cells, although it migrates into the cytoplasm of dividing cells. It was originally identified by as the protein most efficiently UV cross-linked to the A, B and C spliceosomal complexes (1). The protein contains two of the highly conserved RRM type RNA recognition motifs, each corresponding to a small ~70 amino acid structure, consisting of 4 beta strands and two alpha-helices. Proteins containing these proteins are believed to have a role in the regulation of mRNA splicing. The protein runs on SDS-PAGE gels at an apparent molecular weight of 49kD. This protein is known as splicing factor 3b, subunit 4, 49kD SAP49, spliceosome-associated protein (U2 snRNP), Hsh49 and MGC108282. Antibodies to this protein are good markers of nuclei. The HGNC name for this protein is SF3B4.
Catalog #031429
ApplicationsSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:1000
Western Blot: 1:1000 using chemiluminescence
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b,k
Clone No13B859
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 10mM sodium azide.
PurityPurified by affinity chromatography.
ImmunogenFull length recombinant human SF3B4 expressed in and purified from E. coli.
SpecificityRecognizes SF3B4 from human, bovine, porcine, mouse, rat and other mammals. Since the SF3B4 protein sequence is highly conserved across species barriers, it is likely that the antibody is effective on many other species also.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesSplicing Factor 3b Subunit 4 49 kD, Spliceosome-associated Protein (U2 snRNP)


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