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031939 Rabbit Anti-AP1S1, NT (AP1S1, AP19, CLAPS1, AP-1 complex subunit sigma-1A, Adapter-related protein complex 1 sigma-1A subunit, Adaptor protein complex AP-1 sigma-1A subunit, Clathrin assembly protein complex 1 sigma-1A small chain, Clathrin coat assembly protein AP19, Golgi adaptor HA1/AP1 adaptin sigma-1A subunit, HA1 19kD subunit, Sigma 1a subunit of AP-1 clathrin, Sigma-adaptin 1A, Sigma1A-adaptin)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P61966
Isotype
IgG
Grade
Affinity Purified
Applications
E FC IHC WB
Crossreactivity
Hu
Gene ID
1174
Gene #
AP1S1
Shipping Temp
Blue Ice
Storage Temp
-20°C
AP1S1, AP19, CLAPS1, AP-1 complex subunit sigma-1A, Adapter-related protein complex 1 sigma-1A subunit, Adaptor protein complex AP-1 sigma-1A subunit, Clathrin assembly protein complex 1 sigma-1A small chain, Clathrin coat assembly protein AP19, Golgi adaptor HA1/AP1 adaptin sigma-1A subunit, HA1 19kD subunit, Sigma 1a subunit of AP-1 clathrin, Sigma-adaptin 1A, Sigma1A-adaptin

AP1S1 is part of the clathrin coat assembly complex which links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. This protein, as well as beta-prime-adaptin, gamma-adaptin, and the medium (mu) chain AP47, form the AP-1 assembly protein complex located at the Golgi vesicle.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
AP1S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-45 amino acids from the N-terminal region of human AP1S1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human
References
Montpetit, A., et al. PLoS Genet. 4 (12), E1000296 (2008) |Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) |Leong, W.F., et al. Cell. Microbiol. 8(4):565-580(2006)
USBio References
No references available
Conjugates
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