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You are here:Home » Antibodies » Antibodies-Autophagy » Anti -APG10L, CT (S116) (ATG10, Ubiquitin-like-conjugating enzyme ATG10, Autophagy-related protein 10) (Azide free) (HRP)

Anti -APG10L, CT (S116) (ATG10, Ubiquitin-like-conjugating enzyme ATG10, Autophagy-related
protein 10) (Azide free) (HRP)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Catalog #031959-HRP
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended DilutionELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Storage and StabilityStore product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note Applications are based on unconjugated antibody.
Clone TypePolyclonal
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
PurityPurified by Protein A affinity chromatography.
ImmunogenAPG10L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-121 amino acids from the C-terminal region of human APG10L.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesATG10, Ubiquitin-like-conjugating enzyme ATG10, Autophagy-related protein 10

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