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You are here:Home » Antibodies » Abs to Autophagy » Anti -ATG12, NT (ATG12, APG12, APG12L, Ubiquitin-like protein ATG12, Autophagy-related protein 12)

Anti -ATG12, NT (ATG12, APG12, APG12L, Ubiquitin-like protein ATG12, Autophagy-related
protein 12)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E B IH
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG12L is the human homolog of yeast APG12, a ubiquitin-activating enzyme E1-like protein essential for the conjugation system that mediates membrane fusion in autophagy.
Catalog #032170
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended DilutionELISA: 1:1,000
Western Blot: 1:100 (Ref.2)
Immunohistochemistry: 1:50-100
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
ImmunogenATG12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44~73 amino acids from the N-term of human APG12L.
SpecificityHuman
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesATG12, APG12, APG12L, Ubiquitin-like protein ATG12, Autophagy-related protein 12


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