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You are here:Home » Antibodies » Abs to Enzymes, Protease (Proteinase, Peptidase) » Anti -ATG4B (ATG4B, APG4B, AUTL1, KIAA0943, Cysteine protease ATG4B, AUT-like 1 cysteine endopeptidase, Autophagin-1, Autophagy-related cysteine endopeptidase 1, Autophagy-related protein 4 homolog B) (AP)

Anti -ATG4B (ATG4B, APG4B, AUTL1, KIAA0943, Cysteine protease ATG4B, AUT-like 1 cysteine
endopeptidase, Autophagin-1, Autophagy-related cysteine endopeptidase 1, Autophagy-related
protein 4 homolog B) (AP)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4, a cysteine protease required for autophagy, cleaves the C-terminal part of MAP1LC3 to form the activated molecule LC3-I. LC3-I is subsequently conjugated with phosphatidylethanolamine at the C-terminal glycine to form LC3-II, a marker for autophagy via its capacity to bind to autophagosomes.
Catalog #032196-AP
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended DilutionELISA: 1:1,000
Western Blot: 1:50-100
Immunohistochemistry: 1:10-50
Storage and StabilityMay be stored at 4°C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note Applications are based on unconjugated antibody.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Alkaline Phosphatase.
PurityPurified by Protein A affinity chromatography.
ImmunogenATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264~293 amino acids from the central region of human APG4B.
SpecificityHuman
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesATG4B, APG4B, AUTL1, KIAA0943, Cysteine protease ATG4B, AUT-like 1 cysteine endopeptidase, Autophagin-1, Autophagy-related cysteine endopeptidase 1, Autophagy-related protein 4 homolog B


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