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You are here:Home » Antibodies » Antibodies-Clathrin » Anti -ATP6V0A1, NT (ATP6V0A1, ATP6N1, ATP6N1A, VPP1, V-type proton ATPase 116kD subunit a isoform 1, Clathrin-coated vesicle/synaptic vesicle proton pump 116kD subunit, Vacuolar adenosine triphosphatase subunit Ac116, Vacuolar proton pump subunit 1, Vacuolar pr

Anti -ATP6V0A1, NT (ATP6V0A1, ATP6N1, ATP6N1A, VPP1, V-type proton ATPase 116kD subunit a
isoform 1, Clathrin-coated vesicle/synaptic vesicle proton pump 116kD subunit, Vacuolar
adenosine triphosphatase subunit Ac116, Vacuolar proton pump subunit 1, Vacuolar pr

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH FC
ATP6V0A1 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domai.n and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three A subunit proteins and the encoded protein is associated with clathrin-coated vesicles.
Catalog #032291
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Flow Cytometry and ELISA. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1:1000
Flow Cytometry: 1:10-1:50
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenKLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 44-71 in the N-terminal region of human ATP6V0A1, UniProt Accession #Q93050. Species sequence homology: bovine, chicken, mouse, rat, Xenopus, rabbit
SpecificityRecognizes human ATP6V0A1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesATP6V0A1, ATP6N1, ATP6N1A, VPP1, V-type proton ATPase 116kD subunit a isoform 1, Clathrin-coated vesicle/synaptic vesicle proton pump 116kD subunit, Vacuolar adenosine triphosphatase subunit Ac116, Vacuolar proton pump subunit 1, Vacuolar proton transloca


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