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You are here:Home » Antibodies » Abs to Enzymes, ATPase » Anti -ATP6V0B, ID (ATP6V0B, ATP6F, V-type proton ATPase 21kD proteolipid subunit, Vacuolar proton pump 21kD proteolipid subunit, hATPL)

Anti -ATP6V0B, ID (ATP6V0B, ATP6F, V-type proton ATPase 21kD proteolipid subunit, Vacuolar
proton pump 21kD proteolipid subunit, hATPL)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is part of the transmembrane V0 domain and is the human counterpart of yeast VMA16. Two alternatively spliced transcript variants that encode different proteins have been found for this gene.
Catalog #032294
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended DilutionELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenATP6V0B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-139 amino acids from the Central region of human ATP6V0B.
SpecificityHuman
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesATP6V0B, ATP6F, V-type proton ATPase 21kD proteolipid subunit, Vacuolar proton pump 21kD proteolipid subunit, hATPL


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