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You are here:Home » Antibodies » Abs to Mitochondrial Proteins » Anti -Bit1, ID (PTRH2, BIT1, PTH2, Peptidyl-tRNA hydrolase 2, mitochondrial, Bcl-2 inhibitor of transcription 1) (APC)

Anti -Bit1, ID (PTRH2, BIT1, PTH2, Peptidyl-tRNA hydrolase 2, mitochondrial, Bcl-2
inhibitor of transcription 1) (APC)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Adhesion to extracellular matrix regulates cell survival via integrin engagement and cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. A role for Bit1 (Bcl-2 inhibitor of transcription 1) has been identified in this process. Bit1 is a mitochondrial protein released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. Bit1-AES complexes may switch off a survival-promoting gene transcription program controlled by TLE. Apoptosis of Bit1/AES transfected cells is inhibited when cells are permitted to attach to fibronectin through the alpha-beta integrin, suggesting that the contribution of the Bit1-AES pathway to anoikis is regulated by integrins.
Catalog #032527-APC
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, FLISA
Recommended DilutionFLISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note Applications are based on unconjugated antibody.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Allophycocyanin (APC).
PurityPurified by Protein A affinity chromatography.
ImmunogenBit1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88~118 amino acids from the Center region of human Bit1.
SpecificityHuman
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesPTRH2, BIT1, PTH2, Peptidyl-tRNA hydrolase 2, mitochondrial, Bcl-2 inhibitor of transcription 1


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