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033538 Rabbit Anti-CD73 (NT5E), NT (NT5E, NT5, NTE, 5'-nucleotidase, Ecto-5'-nucleotidase, CD73)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P21589
Isotype
IgG
Grade
Purified
Applications
E IF IHC WB
Crossreactivity
Hu
Gene ID
4907
Gene #
NT5E
Shipping Temp
Blue Ice
Storage Temp
-20°C
NT5E, NT5, NTE, 5'-nucleotidase, Ecto-5'-nucleotidase, CD73

Ecto-5-prime-nucleotidase catalyzes conversion at neutral pH of purine 5-prime mononucleotides to nucleosides. The enzyme consists of a dimer of 2 identical 70 kD subunits tethered by a glycosyl phosphatidyl inositol moeity to the exterior plasma membrane surface. The enzyme, a marker of lymphocyte differentiation is associated in deficiency with a variety of immunodeficiency diseases. The preferred substrate is AMP. The NT5 gene has been localized to 6q14-q21 by immunofluorescence and a study of a panel of human x mouse hybrids that contained fragments of chromosome 6 as translocations.

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
CD73 (NT5E) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human NT5E.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Specificity
Human
References
Hashikawa, T., et al., J. Dent. Res. 82(11):888-892 (2003).|Rosi, F., et al., Biomed. Pharmacother. 56(2):100-104 (2002).|Misumi, Y., et al., Eur. J. Biochem. 191(3):563-569 (1990).|Boyle, J.M., et al., Hum. Genet. 81(1):88-92 (1988).|Kalsi, K., et al., Mol. Cell. Biochem. 232 (1-2), 113-119 (2002).
USBio References
No references available
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