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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -CRYAA, ID (CRYAA, CRYA1, HSPB4, Alpha-crystallin A chain, Heat shock protein beta-4, Alpha-crystallin A chain, short form) (Azide free) (HRP)

Anti -CRYAA, ID (CRYAA, CRYA1, HSPB4, Alpha-crystallin A chain, Heat shock protein beta-4,
Alpha-crystallin A chain, short form) (Azide free) (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC). [provided by RefSeq].
Catalog #034251-HRP
ApplicationsSuitable for use in Western Blot, ELISA
Recommended DilutionELISA: 1:1,000
Western Blot: 1:100-500
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2. No preservative added (Azide free). Conjugated to HRP.
PurityPurified by Protein A affinity chromatography.
ImmunogenCRYAA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-106 amino acids from the Central region of human CRYAA.
SpecificityHuman
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCRYAA, CRYA1, HSPB4, Alpha-crystallin A chain, Heat shock protein beta-4, Alpha-crystallin A chain, short form


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