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034477 Rabbit Anti-DARS, NT (DARS, Aspartate-tRNA ligase, cytoplasmic, Aspartyl-tRNA synthetase, Cell proliferation-inducing gene 40 protein)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P14868
Isotype
IgG
Grade
Purified
Applications
E FC IF IHC WB
Crossreactivity
Hu
Accession #
NP_001340.2
Gene ID
1615
Gene #
DARS
Shipping Temp
Blue Ice
Storage Temp
-20°C
DARS, Aspartate--tRNA ligase, cytoplasmic, Aspartyl-tRNA synthetase, Cell proliferation-inducing gene 40 protein

Aspartyl-tRNA synthetase (DARS) is part of a multienzyme complex of aminoacyl-tRNA synthetases. Aspartyl-tRNA synthetase charges its cognate tRNA with aspartate during protein biosynthesis.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-100 Immunohistochemistry: 1:50-100 Immunofluorescence: 1:10-50 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
DARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-190 amino acids from the N-terminal region of human DARS.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Human
References
Wu, C., et al. Proteomics 7(11):1775-1785(2007)|Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)|Tu, L.C., et al. Mol. Cell Proteomics 6(4):575-588(2007)|Ling, C., et al. J. Biol. Chem. 280(41):34755-34763(2005)|Bonnefond, L., et al. Biochemistry 44(12):4805-4816(2005)
USBio References
No references available
Conjugates
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