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034517 Rabbit Anti-DCT, NT (DCT, TYRP2, L-dopachrome tautomerase, L-dopachrome Delta-isomerase, Tyrosinase-related protein 2)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P40126
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Gene ID
1638
Gene #
DCT
Shipping Temp
Blue Ice
Storage Temp
-20°C
DCT, TYRP2, L-dopachrome tautomerase, L-dopachrome Delta-isomerase, Tyrosinase-related protein 2

Involved in regulating eumelanin and phaeomelanin levels. The tyrosinase family in vertebrates consists of three related melanogenic enzymes: tryrosinase, tyrosinase-related protein-1 (TRP-1) and tyrosinase-related protein-2 (TRP-2). These proteins control melanin production in pigment cells and play a crucial role in determining vertebrate coloration and are expressed by melanoma cells, normal melanocytes and nevi, but not carcinomas or sarcomas.

Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 62-89 in the N-terminal region of human DCT, UniProt Accession #P40126.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human DCT.
References
1. Edwards, M., et al. PLoS Genet. 6 (3), E1000867 (2010). 2. Khong, H.T., et al. J. Immunol. 168(2):951-956(2002). 3. Sugimoto, H., et al. Biochemistry 38(11):3268-3279(1999)
USBio References
No references available
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