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034887 Rabbit Anti-E2F1, phosphorylated (H357) (E2F1, RBBP3, Transcription factor E2F1, PBR3, PRB-binding protein E2F-1, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
Q01094
Isotype
IgG
Grade
Affinity Purified
Applications
DB E WB
Crossreactivity
Hu
Gene ID
1869
Gene #
E2F1
Shipping Temp
Blue Ice
Storage Temp
-20°C
E2F1, RBBP3, Transcription factor E2F1, PBR3, PRB-binding protein E2F-1, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Applications
Suitable for use in Dot Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Phospho-E2F1-H357 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding H357 of human E2F1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human
References
Pulikkan, J.A., et al. Blood 115(9):1768-1778(2010)|Paik, J.C., et al. J. Biol. Chem. 285(9):6348-6363(2010)|Alla, V., et al. J. Natl. Cancer Inst. 102(2):127-133(2010)|Zhou, C., et al. Mol. Endocrinol. 23(12):2000-2012(2009)|Yang, X., et al. Genes Dev. 23(20):2388-2393(2009)|Olsen, J.V., et al. Cell 127(3):635-648(2006)
USBio References
No references available
Conjugates
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