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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -EIF2S1, NT (EIF2S1, EIF2A, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha)

Anti -EIF2S1, NT (EIF2S1, EIF2A, Eukaryotic translation initiation factor 2 subunit 1,
Eukaryotic translation initiation factor 2 subunit alpha)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH IF
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).
Catalog #034996
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Recommended DilutionELISA: 1:1,000
Western Blot: 1:100-500
Immunofluorescence: 1:10-50
Immunohistochemistry: 1:10-50
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenEIF2S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-89 amino acids from the N-terminal region of human EIF2S1.
SpecificityHuman, mouse (predicted)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEIF2S1, EIF2A, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha


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