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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -EIF4E (EIF4E, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4F 25kD subunit, mRNA cap-binding protein)

Anti -EIF4E (EIF4E, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4F
25kD subunit, mRNA cap-binding protein)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH FC
EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.
Catalog #035012
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Recommended DilutionELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenEIF4E antibody is generated from rabbits immunized with a recombinant protein from human VGFR2.
SpecificityHuman
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEIF4E, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4F 25kD subunit, mRNA cap-binding protein


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