EREG, CT

Clone Type

Polyclonal

Host

Rabbit

Source

Human

Swiss Prot

O14944

Isotype

IgG

Grade

Affinity Purified

Applications

E IHC WB

Crossreactivity

Gene ID

2069

Gene #

EREG

Shipping Temp

Blue Ice

Storage Temp

-20°C

EREG, Proepiregulin, Epiregulin

EREG is a member of the epidermal growth factor family. EREG can function as a ligand of EGFR (epidermal growth factor receptor), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors.

Applications

Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested.

Recommended Dilution

Western Blot: 1:1000-1:2000 Immunohistochemistry (FFPE): 1:10-1:50 Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 137-165 in the C-terminal region of human EREG.

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity

Purified by Protein A and peptide affinity chromatography.

Specificity

Recognizes human EREG.

References

1. Ben-Ami, I., et al. Hum. Reprod. 24(1):176-184(2009) 2. Cho, M.C., et al. Biochem. Biophys. Res. Commun. 377(3):832-837(2008) 3. Lasky-Su, J., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (8), 1345-1354 (2008)