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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP

Epitope tags are useful for the labeling and detection of recombinant proteins using western blotting, immunoprecipitation and immunostaining techniques. The eight amino acid DYKDDDDK peptide is an established and multi-functional epitope tag and can be expressed and detected with a recombinant protein as an amino-terminal or carboxy-terminal fusion (1). Abgent?s DYKDDDDK antibody binds to the same epitope recognized by Sigma's Anti-FLAG?antibodies. (FLAG?is a registered trademark of Sigma-Aldrich Co., which is not affiliated with Abgent).
Catalog #	035671
Applications	Suitable for use in Western Blot, ELISA
Recommended Dilution	ELISA: 1:5,000
	Western Blot: 1:50-100
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	0.25mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	KLH conjugated synthetic peptide (CDYKDDDDKDYKDDDDK) was used as antigen.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	Tag from influenza hemagglutinin protein