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You are here:Home » Antibodies » Abs to Histones » Anti -H4 ( K20 [Me2] ) (HIST1H4A, H4/A, H4FA, Histone H4)

Anti -H4 ( K20 [Me2] ) (HIST1H4A, H4/A, H4FA, Histone H4)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E IH FC
Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones.
Catalog #036410
ApplicationsSuitable for use in Immunohistochemistry, Flow Cytometry, Dot Blot, ELISA
Recommended DilutionELISA: 1:1,000
Dot blot 1:500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenH4 antibody is generated from rabbits immunized with a KLH conjugated synthetic methylation peptide between 4~33 amino acids surrounding K20(Methyl 2) of human H4.
SpecificityHuman
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesHIST1H4A, H4/A, H4FA, Histone H4


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