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You are here:Home » Antibodies » Antibodies-Histones » Anti -H4 ( K20 [Me2] ) (HIST1H4A, H4/A, H4FA, Histone H4)

Anti -H4 ( K20 [Me2] ) (HIST1H4A, H4/A, H4FA, Histone H4)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH FC
Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones.
Catalog #036410
ApplicationsSuitable for use in Immunohistochemistry, Flow Cytometry, Western Blot, Dot Blot and ELISA. Other applications have not been tested.
Recommended DilutionsDot Blot: 1:500
Western Blot: 1:1000
Immunohistochemistry (FFPE): 1:50-1:100
Flow Cytometry: 1:10-1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
ConcentrationAs Reported
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenKLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 of human H4, UniProt Accession #P62805.
SpecificityRecognizes human dimethylated (K20) histone H4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesHIST1H4A, H4/A, H4FA, Histone H4

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