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You are here:Home » Antibodies » Abs to Histones » Anti -HIST1H2AL, CT (HIST1H2AG, H2AFP, Histone H2A type 1, Histone H2A/p)

Anti -HIST1H2AL, CT (HIST1H2AG, H2AFP, Histone H2A type 1, Histone H2A/p)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH FC
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.
Catalog #036590
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000
Immunohistochemistry (FFPE): 1:50-1:100
Flow Cytometry: 1:10-1:50
Optimal dilutions to be geterined by the researcher.
Positive ControlHL-60, HepG2, K562, CEM, MCF-7 cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs Reported
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to a portion of amino acids within aa103-130 at the C-terminal of human HIST1H2AL, conjugated to KLH.
Cellular LocalizationNucleus, Chromosome. Species sequence homology: bovine, mouse and rat
SpecificityRecognizes human HIST1H2AL at ~14kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesHIST1H2AG, H2AFP, Histone H2A type 1, Histone H2A/p


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