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036590 Rabbit Anti-HIST1H2AL, CT (HIST1H2AG, H2AFP, Histone H2A type 1, Histone H2A/p)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P0C0S8
Isotype
IgG
Grade
Affinity Purified
Applications
FC IHC WB
Crossreactivity
Hu
Gene ID
8969
Gene #
HIST1H2AL; HIST1H2AI; HIST1H2AK; HIST1H2AM; HIST1H
Shipping Temp
Blue Ice
Storage Temp
-20°C
HIST1H2AG, H2AFP, Histone H2A type 1, Histone H2A/p

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

Applications
Suitable for use in Western Blot, Immunohistochemistry and Indirect Flow Cytometry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:50-1:100 Indirect Flow Cytometry: 1:10-1:50 Optimal dilutions to be geterined by the researcher.
Positive Control
HL-60, HepG2, K562, CEM, MCF-7 cell lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa103-130 in the C-terminal region of human HIST1H2AL, UniProt Accession #P0C0S8|Cellular Localization: Nucleus, Chromosome. Species sequence homology: bovine, mouse and rat
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human HIST1H2AL at ~14kD.
References
1. Lusic, M., et al. EMBO J. 22(24):6550-6561(2003). 2. Kzhyshkowska, J., et al. Biochem. J. 371 (PT 2), 385-393 (2003). 3. Marzluff, W.F., et al. Genomics 80(5):487-498(2002)
USBio References
No references available
Conjugates
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