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036632 Rabbit Anti-HMFG, CT (MFGE8, Lactadherin, Breast epithelial antigen BA46, HMFG, MFGM, Milk fat globule-EGF factor 8, SED1, Lactadherin short form, Medin)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
Q08431
Isotype
IgG
Grade
Affinity Purified
Applications
E FC WB
Crossreactivity
Hu
Accession #
NP_005919.2; NP_001108086.1
Gene ID
4240
Gene #
MFGE8
Shipping Temp
Blue Ice
Storage Temp
-20°C
MFGE8, Lactadherin, Breast epithelial antigen BA46, HMFG, MFGM, Milk fat globule-EGF factor 8, SED1, Lactadherin short form, Medin

HMFG plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine- enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. Medin is the main constituent of aortic medial amyloid.

Applications
Suitable for use in ELISA, Western Blot and Indirect Flow Cytometry. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:1000 Indirect Flow Cytometry: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 316-345 in the C-terminal region of human HMFG.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human HMFG.
References
1. Yamaguchi, H., et al. Eur. J. Immunol. 40(6):1778-1785(2010). 2. Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009). 3. Hu, C.Y., et al. Lupus 18(8):676-681(2009). 4. Fu, Z., et al. Circ. Res. 104(12):1337-1346(2009). 5. Raymond, A., et al. J. Cell. Biochem. 106(6):957-966(2009)
USBio References
No references available
Conjugates
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