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036779 Rabbit Anti-HSD17B12, ID (HSD17B12, Estradiol 17-beta-dehydrogenase 12, 17-beta-hydroxysteroid dehydrogenase 12, 3-ketoacyl-CoA reductase)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
Q53GQ0
Isotype
IgG
Grade
Affinity Purified
Applications
E FC IHC WB
Crossreactivity
Hu
Gene ID
51144
Gene #
HSD17B12
Shipping Temp
Blue Ice
Storage Temp
-20°C
HSD17B12, Estradiol 17-beta-dehydrogenase 12, 17-beta-hydroxysteroid dehydrogenase 12, 3-ketoacyl-CoA reductase

The enzyme 17-beta hydroxysteroid dehydrogenase-12 (HSD17B12) uses NADPH to reduce 3-ketoacyl-CoA to 3-hydroxyacyl-CoA during the second step of fatty acid elongation.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry and ELISA. Other applications have not been tested.
Recommended Dilution
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:10-1:50 Indirect Flow Cytometry: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 126-155 in the central region of human HSD17B12. Species sequence homology: monkey
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human HSD17B12.
References
Plourde,M. J. Steroid Biochem. Mol. Biol. 116 (3-5), 134-153 (2009) Nagasaki,S. Mol. Cell. Endocrinol. 307 (1-2), 163-168 (2009) Bellemare,V. J. Steroid Biochem. Mol. Biol. 114 (3-5), 129-134 (2009)
USBio References
No references available
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