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037021 Rabbit Anti-ILK1/ILK2, NT (Ilk, Integrin-linked protein kinase)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
O55222
Isotype
IgG
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu
Gene ID
16202
Gene #
Ilk
Shipping Temp
Blue Ice
Storage Temp
-20°C
Ilk, Integrin-linked protein kinase

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. ILK encodes a predicted 451-amino acid protein, with an apparent molecular weight of 59kD. The ILK protein is a serine/threonine protein kinase with 4 ankyrin-like repeats. ILK regulates integrin-mediated signal transduction.

Applications
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 11-41 in the N-terminal region of human ILK1/ILK2. Species Sequence Homology: bovine, chicken and rat.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human ILK1/ILK2.
References
1. Li, Y., et al., J. Clin. Invest. 112(4):503-516 (2003). 2. Troussard, A.A., et al., J. Biol. Chem. 278(25):22374-22378 (2003). 3. Marotta, A., et al., Br. J. Cancer 88(11):1755-1762 (2003). 4. Cordes, N., et al., Br. J. Cancer 88(9):1470-1479 (2003). 5. Fukuda, T., et al., J. Cell Biol. 160(7):1001-1008 (2003).
USBio References
No references available
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