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You are here:Home » Antibodies » Abs to Enzymes, Demethylase » Anti -JHDM1D, ID (JHDM1D, KDM7, KIAA1718, Lysine-specific demethylase 7, JmjC domain-containing histone demethylation protein 1D) (APC)

Anti -JHDM1D, ID (JHDM1D, KDM7, KIAA1718, Lysine-specific demethylase 7, JmjC
domain-containing histone demethylation protein 1D) (APC)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH FC
Catalog #037191-APC
JHDM1D histone demethylase that specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3, thereby playing a central role in histone code. This specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. This demethylates H3K9me2 in absence of H3K4me3.
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, FLISA
Recommended DilutionFLISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note Applications are based on unconjugated antibody.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Allophycocyanin (APC).
PurityPurified by Protein A affinity chromatography.
ImmunogenJHDM1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 295-325 amino acids from the Central region of human JHDM1D.
SpecificityHuman
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesJHDM1D, KDM7, KIAA1718, Lysine-specific demethylase 7, JmjC domain-containing histone demethylation protein 1D


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