MID2, CT

Clone Type

Polyclonal

Host

Rabbit

Source

Human

Swiss Prot

Q9UJV3

Isotype

IgG

Grade

Affinity Purified

Applications

E IHC WB

Crossreactivity

Accession #

NP_036348.2; NP_438112.2

Gene ID

11043

Gene #

MID2

Shipping Temp

Blue Ice

Storage Temp

-20°C

MID2, FXY2, RNF60, TRIM1, Probable E3 ubiquitin-protein ligase MID2, Midin-2, Midline defect 2, Midline-2, RING finger protein 60, Tripartite motif-containing protein 1

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms.

Applications

Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.

Recommended Dilutions

Immunohistochemistry: Formalin fixed, paraffin embedded tissues Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 690-717 in the C-terminal region of human MID2. Species Sequence Homology: mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity

Purified by Protein A and peptide affinity chromatography.

Specificity

Recognizes human MID2.

References

1. Lim, J., et al. Cell 125(4):801-814(2006). 2. Jehee, F.S., et al. Am. J. Med. Genet. A 139(3):221-226(2005). 3. Yap, M.W., et al. Proc. Natl. Acad. Sci. U.S.A. 101(29):10786-10791(2004). 4. Short, K.M., et al. BMC Cell Biol. 3, 1 (2002). 5. Reymond, A., et al. EMBO J. 20(9):2140-2151(2001)

USBio References

No references available

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038429 Rabbit Anti-MID2, CT (MID2, FXY2, RNF60, TRIM1, Probable E3 ubiquitin-protein ligase MID2, Midin-2, Midline defect 2, Midline-2, RING finger protein 60, Tripartite motif-containing protein 1)

Clone Type

Host

Source

Swiss Prot

Isotype

Grade

Applications

Crossreactivity

Accession #

Gene ID

Gene #

Shipping Temp

Storage Temp

MID2, FXY2, RNF60, TRIM1, Probable E3 ubiquitin-protein ligase MID2, Midin-2, Midline defect 2, Midline-2, RING finger protein 60, Tripartite motif-containing protein 1

References

USBio References

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