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038951-APC Rabbit Anti-NFYC, NT (NFYC, Nuclear transcription factor Y subunit gamma, CAAT box DNA-binding protein subunit C, Nuclear transcription factor Y subunit C, Transactivator HSM-1/2) (APC)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
Q13952
Conjugate
APC
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA WB
Crossreactivity
Hu
Accession #
NP_055038.2; NP_001136059.1; NP_001136062.1
Gene ID
4802
Gene #
NFYC
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
NFYC, Nuclear transcription factor Y subunit gamma, CAAT box DNA-binding protein subunit C, Nuclear transcription factor Y subunit C, Transactivator HSM-1/2

This gene encodes one subunit of a trimeric complex forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoters of a variety of genes. The encoded protein, subunit C, forms a tight dimer with the B subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Multiple transcript variants encoding different isoforms have been found for this gene.

Applications
Suitable for use in Western Blot and FLISA. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 100-129 in the N-terminal region of human NFYC. Species Sequence Homology: bovine, mouse and rat
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human NFYC.
References
1. Ichikawa, S., et al. J. Bone Miner. Res. 25(8):1821-1829(2010). 2. Murai-Takeda, A., et al. J. Biol. Chem. 285(11):8084-8093(2010). 3. Ceribelli, M., et al. J. Biol. Chem. 284(49):34189-34200(2009). 4. Yamanaka, K., et al. Genomics 94(4):219-227(2009). 5. Horikawa, Y., et al. Clin. Cancer Res. 14(23):7956-7962(2008)
USBio References
No references available
Conjugates
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