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038981 Rabbit Anti-NIX, ID (BNIP3L, BNIP3A, BNIP3H, NIX, BCL2/adenovirus E1B 19kD protein-interacting protein 3-like, Adenovirus E1B19K-binding protein B5, BCL2/adenovirus E1B 19kD protein-interacting protein 3A, NIP3-like protein X)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
O60238
Isotype
IgG
Grade
Affinity Purified
Applications
E FC IF IHC WB
Crossreactivity
Hu
Accession #
NP_004322.1
Gene ID
665
Gene #
BNIP3L
Shipping Temp
Blue Ice
Storage Temp
-20°C
BNIP3L, BNIP3A, BNIP3H, NIX, BCL2/adenovirus E1B 19kD protein-interacting protein 3-like, Adenovirus E1B19K-binding protein B5, BCL2/adenovirus E1B 19kD protein-interacting protein 3A, NIP3-like protein X

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. The protein encoded by this gene is a functional homolog of BNIP3, a proapoptotic protein. This protein may function simultaneously with BNIP3 and may play a role in tumor suppression.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Indirect Flow Cytometry and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:50-1:100 Immunofluorescence: 1:10-1:50 Indirect Flow Cytometry: 1:10-1:0 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 52-81 in the centralterminal region of human NIX. Species Sequence Homology: bovine and mouse
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human NIX
References
1. Novak, I., et al. EMBO Rep. 11(1):45-51(2010). 2. Bellot, G., et al. Mol. Cell. Biol. 29(10):2570-2581(2009). 3. Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3558-3566(2008). 4. Papandreou, I., et al. Cell Death Differ. 15(10):1572-1581(2008).5. Liu, W., et al. Neoplasia 10(8):897-907(2008)
USBio References
No references available
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