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039072 Rabbit Anti-NOX4, NT (NOX4, RENOX, NADPH oxidase 4, Kidney oxidase-1, Kidney superoxide-producing NADPH oxidase, Renal NAD(P)H-oxidase)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
Q9NPH5
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_001137308.1; NP_058627.1
Gene ID
50507
Gene #
NOX4
Shipping Temp
Blue Ice
Storage Temp
-20°C
NOX4, RENOX, NADPH oxidase 4, Kidney oxidase-1, Kidney superoxide-producing NADPH oxidase, Renal NAD(P)H-oxidase

This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.

Applications
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:2000 Immunohistochemistry (FFPE): 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 124-153 in the N-terminal region of human NOX4, UniProt Accession #Q9NPH5.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Purity
Purified by Protein A and peptide affinity chromatography
Specificity
Recognizes human NOX4.
References
1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253 (2010). 2. Salmeen, A., et al. Oncogene 29(31):4473-4484 (2010). 3. Wu, R.F., et al. Mol. Cell. Biol. 30(14):3553-3568 (2010). 4. Diebold, I., et al. Mol. Biol. Cell 21(12):2087-2096 (2010). 5. Manea, A., et al. Biochem. Biophys. Res. Commun. 396(4):901-907 (2010)
USBio References
No references available
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