OPN-a/b, NT

Clone Type

Polyclonal

Host

Rabbit

Source

Human

Swiss Prot

P10451

Isotype

IgG

Grade

Affinity Purified

Applications

E IF IHC WB

Crossreactivity

Accession #

NP_000573.1; NP_001035147.1

Gene ID

6696

Gene #

SPP1

Shipping Temp

Blue Ice

Storage Temp

-20°C

SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity).

Applications

Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA. Other applications not tested.

Recommended Dilutions

Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:50-1:100 Immunofluorescence: 1:25 Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 14-40 in the N-terminal region of human OPN-a/b, UniProt Accession #P10451.

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity

Purified by Protein A and peptide affinity chromatography.

Specificity

Recognizes human OPN-a/b.

References

1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) 2. Weber, G.F., et al. Br. J. Cancer 103(6):861-869(2010) 3. Chen, R.X., et al. J. Gastroenterol. Hepatol. 25(8):1435-1442(2010) 4. Jugessur, A., et al. PLoS ONE 5(7), E11493 (2010) 5. Johnatty, S.E., et al. PLoS Genet. 6(7), E1001016 (2010)

USBio References

No references available

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039288 Rabbit Anti-OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin)

Clone Type

Host

Source

Swiss Prot

Isotype

Grade

Applications

Crossreactivity

Accession #

Gene ID

Gene #

Shipping Temp

Storage Temp

SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin

References

USBio References

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