PAP, ID

Clone Type

Polyclonal

Host

Rabbit

Source

Human

Swiss Prot

P51003

Isotype

IgG

Grade

Affinity Purified

Applications

E IHC WB

Crossreactivity

Accession #

NP_116021.2

Gene ID

10914

Gene #

PAPOLA

Shipping Temp

Blue Ice

Storage Temp

-20°C

PAPOLA, PAP, Poly(A) polymerase alpha, Polynucleotide adenylyltransferase alpha

Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.

Applications

Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested.

Recommended Dilution

Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:50-1:100 Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 407-435 in the central region of human PAP, UniProt Accession #P51003.Species sequence homology: bovine

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity

Purified by Protein A and peptide affinity chromatography.

Specificity

Recognizes human PAP.

References

1. Rapti, A., et al. Mol. Cell. Biochem. 340 (1-2), 91-96 (2010) : 2. Nan, H., et al. J. Invest. Dermatol. 129(9):2250-2257(2009) 3. Olsen, J.V., et al. Cell 127(3):635-648(2006) 4. Dettwiler, S., et al. J. Biol. Chem. 279(34):35788-35797(2004) 5. Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)

USBio References

No references available

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039641 Rabbit Anti-PAP, ID (PAPOLA, PAP, Poly(A) polymerase alpha, Polynucleotide adenylyltransferase alpha)

Clone Type

Host

Source

Swiss Prot

Isotype

Grade

Applications

Crossreactivity

Accession #

Gene ID

Gene #

Shipping Temp

Storage Temp

PAPOLA, PAP, Poly(A) polymerase alpha, Polynucleotide adenylyltransferase alpha

References

USBio References

Conjugates

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