PYGO1 is involved in signal transduction through the Wnt pathway.
Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50 Immunofluorescence: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PYGO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-417 amino acids from the C-terminal region of human PYGO1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human
References
Frank, B., et al. Carcinogenesis (2010) In press :|Fiedler, M., et al. Mol. Cell 30(4):507-518(2008)|Townsley, F.M., et al. J. Biol. Chem. 279(7):5177-5183(2004)|Thompson, B., et al. Nat. Cell Biol. 4(5):367-373(2002)|Kramps, T., et al. Cell 109(1):47-60(2002)