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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -SMAD2, phosphorylated (T220) (SMAD2, MADH2, MADR2, Mothers against decapentaplegic homolog 2, JV18-1, Mad-related protein 2, SMAD family member 2)

Anti -SMAD2, phosphorylated (T220) (SMAD2, MADH2, MADR2, Mothers against decapentaplegic
homolog 2, JV18-1, Mad-related protein 2, SMAD family member 2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E IF Gel
SMAD2 is a 58 kDa member of a family of proteins involved in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor-regulated Smad's, activin/TGF?receptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smad's; and the inhibitory Smad's, (Smad6 and Smad7). Smad2 consists of two highly conserved domains, the N terminal Mad homology (MH1) and the C-terminal Mad homology 2 (MH2) domains. The MH1 domain binds DNA and regulates nuclear import and transcription while the MH2 domain conserved among all the Smad's regulates Smad2 oligomerization and binding to cytoplasmic adaptors and transcription factors. Activated Smad2 associates with Smad4 and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. This translocation of Smad2 (as well as Smad3) into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin binding region of the MH1 domain by extracellular signal regulated kinase 1(ERK 1) enhances Smad2 transcriptional activity, which is negatively regulated by calmodulin. The regulation of Smad2 phosphorylation on threonine 8 by ERK 1 and calmodulin is critical for Smad2 mediated signaling.
Catalog #042020
ApplicationsSuitable for use in Immunofluorescence, Dot Blot, ELISA
Recommended DilutionELISA: 1:1,000
Dot blot 1:500
Immunofluorescence: 1:10-50
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenPhospho-SMAD2-T220 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 208~238 amino acids surrounding T220 of human SMAD2.
SpecificityHuman
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesSMAD2, MADH2, MADR2, Mothers against decapentaplegic homolog 2, JV18-1, Mad-related protein 2, SMAD family member 2


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