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042196 Rabbit Anti-SPAG8, CT (SPAG8, Sperm-associated antigen 8, HSD-1, Sperm membrane protein 1, Sperm membrane protein BS-84)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
Q99932
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_758516.1; NP_001034681.1
Gene ID
26206
Gene #
SPAG8
Shipping Temp
Blue Ice
Storage Temp
-20°C
SPAG8, Sperm-associated antigen 8, HSD-1, Sperm membrane protein 1, Sperm membrane protein BS-84

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
SPAG8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 390-419 amino acids from the C-terminal region of human SPAG8.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human
References
Wu, H., et al. FEBS Lett. 584(13):2807-2815(2010)|Li, R., et al. Cell Biochem. Funct. 27(5):264-268(2009)|Cheng, G.Y., et al. Asian J. Androl. 9(1):23-29(2007)|Tang, X., et al. J. Mol. Med. 82(6):383-388(2004)|Humphray, S.J., et al. Nature 429(6990):369-374(2004)
USBio References
No references available
Conjugates
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