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You are here:Home » Antibodies » Abs to Topoisomerases » Anti -TOP2A, CT (TOP2A, TOP2, DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme) (Azide free) (HRP)

Anti -TOP2A, CT (TOP2A, TOP2, DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha
isozyme) (Azide free) (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH IF
TOP2A encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA.
Catalog #043128-HRP
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Recommended DilutionELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Immunofluorescence: 1:10-50
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Note Applications are based on unconjugated antibody.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2. No preservative added (Azide free). Conjugated to HRP.
PurityPurified by Protein A affinity chromatography.
ImmunogenTOP2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1174~1204 amino acids from the C-terminal region of human TOP2A.
SpecificityHuman
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesTOP2A, TOP2, DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme


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