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043435 Rabbit Anti-TTL, CT (TTL, Tubulin-tyrosine ligase)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
Q8NG68
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_714923.1
Gene ID
150465
Gene #
TTL
Shipping Temp
Blue Ice
Storage Temp
-20°C
TTL, Tubulin--tyrosine ligase

TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination (Erck et al., 2003 [PubMed 14571137]).

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
TTL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 355-385 amino acids from the C-terminal region of human TTL.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human
References
Dick, D.M., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (6), 1179-1188 (2010) :|Erck, C., et al. Cytogenet. Genome Res. 101(1):47-53(2003)|Mialhe, A., et al. Cancer Res. 61(13):5024-5027(2001)|Ersfeld, K., et al. J. Cell Biol. 120(3):725-732(1993)
USBio References
No references available
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