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044086 Rabbit Anti-ZIC3, ID (ZIC3, ZNF203, Zinc finger protein ZIC 3, Zinc finger protein 203, Zinc finger protein of the cerebellum 3)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E FC IHC WB
Crossreactivity
Hu
Accession #
O60481; NP_003404.1
Gene ID
7547
Gene #
ZIC3
Shipping Temp
Blue Ice
Storage Temp
-20°C
ZIC3, ZNF203, Zinc finger protein ZIC 3, Zinc finger protein 203, Zinc finger protein of the cerebellum 3

This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This nuclear protein probably functions as a transcription factor in early stages of left-right body axis formation. Mutations in this gene cause X-linked visceral heterotaxy, which includes congenital heart disease and left-right axis defects in organs.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-100 Immunohistochemistry: 1:50-100 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
ZIC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 246-276 amino acids from the Central region of human ZIC3.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Human
References
Hatayama, M., et al. Hum. Mol. Genet. 17(22):3459-3473(2008)|Zhu, L., et al. Hum. Mutat. 29(1):99-105(2008)|Zhu, L., et al. Hum. Mol. Genet. 16(14):1649-1660(2007)|Chhin, B., et al. Hum. Mutat. 28(6):563-570(2007)|Bedard, J.E., et al. Hum. Mol. Genet. 16(2):187-198(2007)|Zhu, H., et al. Am. J. Med. Genet. A 116A (4), 414-415 (2003) :|Koyabu, Y., et al. J. Biol. Chem. 276(10):6889-6892(2001)|Klootwijk, R., et al. Hum. Mol. Genet. 9(11):1615-1622(2000)|Gebbia, M., et al. Nat. Genet. 17(3):305-308(1997)|Casey, B., et al. Nat. Genet. 5(4):403-407(1993)
USBio References
No references available
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