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You are here:Home » Antibodies » Abs to Blood Groups » Anti -HMGB1 (Recombinant) (Biotin) (High Mobility Group Protein B1)

Anti -HMGB1 (Recombinant) (Biotin) (High Mobility Group Protein B1)

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Specifications

Clone Host Grade Applications
Monoclonal Human Highly Purified E B
HMGB1 was originally discovered as an essential DNA-binding protein for regulating p53, NF-kappaB and other important proteins. It is secreted from activated dentric cells, macrophage and nectrotic cells, and acts as a ligand for RAGE, TLR-2 and TLR-4 expressed on surrounding cells. As a result, HMGB1 activates Rac, CDC42 and NF-kappaB inducing localized innate immunity of damaged tissue, tissue regeneration by recruitment of stem cells and hemostasis by induction of tissue factor expression. HMGB1 is also a causative agent of various diseases as it causes localized inflammation such as arteriosclerosis, chronic rheumatoid arthritis and nephritis.
Catalog #044885
Anti-HMGB1, mAb (recombinant) (Giby-1-4) (Biotin) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
ApplicationsSuitable for use in ELISA, Western Blot
Recommended DilutionOptimal dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2,l
Clone NoGiby-1-4
HostHuman
SourceHuman
Concentration1mg/ml
FormSupplied as a liquid in PBS, 10% glycerol, 0.02% sodium azide.
PurityPurified from HEK 293 cell culture supernatant (>95% (SDS-PAGE).
ImmunogenHuman recombinant HMGB1.
SpecificityRecognizes human, mouse and rat HMGB1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesHigh Mobility Group Protein B1


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