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You are here:Home » Antibodies » Abs to Enzymes, Oxidase » Anti -Indoleamine 2,3-Dioxygenase (IDO, CD107B, INDO, Indoleamine-pyrrole 2,3-dioxygenase)

Anti -Indoleamine 2,3-Dioxygenase (IDO, CD107B, INDO, Indoleamine-pyrrole 2,3-dioxygenase)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IC FC
IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-gamma induce the expression of IDO in several tissues. IDO-dependent suppression of T-cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T-cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.
Catalog #044892
ApplicationsSuitable for use in ELISA, Flow Cytometry, Immunocytochemistry and Western Blot. Other applications not tested.
Recommended DilutionELISA (Direct and Indirect): 1:2000-1:10,000
Western Blot: 1:2000-1:5000 using ECL; Suggested blocking and dilution buffer is PBST containing 0.05% Tween-20 and 5% skim milk; Suggested incubation time is 1 hour at RT.
Flow Cytometry: 1:200-1:500
Immunocytochemistry: 1:500
Optimal dilutions to be determined by the researcher.
Optimal dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration1mg/ml
FormSupplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant human IDO.
SpecificityRecognizes human IDO. Detects a band of ~45kD by Western blot.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesIndoleamine 2,3-dioxygenase, INDO


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