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You are here:Home » Biochemicals » Biochem-Apoptosis » JC-1 (5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine Iodide)

JC-1 (5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine Iodide)

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Specifications

The membrane-permeant dual-emission potential-sensitive JC-1 dye is widely used in apoptosis studies to monitor mitochondrial health by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. JC-1 dye can be used as an indicator of mitochondrial membrane potential in a variety of cell types, including myocytes and neurons, as well as in intact tissues and isolated mitochondria. JC-1 accumulates in mitochondria, selectively generating an orange J-aggregate emission profile (590 nm) in healthy cells. After cell injury, as membrane potential decreases, JC-1 monomers are generated, resulting in a shift to green emission (529 nm). The principal advantage of JC-1 relative to other commonly employed fluorescent probes of mitochondrial membrane potential is that it allows qualitative visualization, considering the shift from orange to green fluorescence emission, and quantitative detection, considering the fluorescence intensity ratio.
Catalog #044974
SolubilityDMSO, Ethanol, Methanol, Acetone
Method for Determining IdentityProton NMR and C-13 NMR
Storage and StabilityProtect from light and moisture.Protect from light. May be stored at 4°C for short-term only. For long-term storage at -20°C. Stable for 12 months after receipt when stored at -20°C.
CAS Number3520-43-2 and 47729-63-5
Molecular FormulaC25H27Cl4IN4
Molecular Weight652.20
Purity>98% (NMR)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide


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