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You are here:Home » Biochemicals » Biochem-Inflammation » Monosodium urate (MSU), ready-to-use

Monosodium urate (MSU), ready-to-use

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NLRP3/NALP3 inflammasome activator. Specially crystallized and tested for biological activity. Stimulates the caspase-1 activating NLRP3/NALP3 inflammasome, resulting in the production of active IL-1beta and IL-18.
Catalog #045039
Activation of THP-1 cells by uric acid1. THP-1 cells are cultured in RPMI 1640 supplemented with 10% heat inactivated fetal bovine
serum (FCS), 50uM -mercaptoethanol, 2mM L-glutamine and penicillin/streptomycin. THP-1 cells are grown in suspension to a density of 1.5x10e6 cells/ml in tissue culture flasks.
2. Treat THP-1 cells for 3 hours with 0.5uM PMA the day before stimulation.
3. Wash cells with PBS and plate them in a 12-well plate at a density of 6x10e5 cells/well in RPMI medium.
4. The next day, wash cells with PBS and add 500ul Optimem (Invitrogen).
5. Stimulate the cells for 6 hours by addition of 50-300ug/ml of the suspension of uric acid (vortex the suspension just before use).
6. As a positive control, 5mM of ATP is incubated for 6 hours.
Detection of the mature Caspase-1 in the supernatant of uric acid-activated THP-1 cells1. Collect the supernatant of each well (500ul). Add 500ul methanol and 125ul chloroform.
2. Vortex and centrifuge for 5 min at 13,000 rpm.
3. Remove the upper phase.
4. Add 500ul methanol and centrifuge for 5 min at 13,000 rpm.
5. Remove the supernatant, dry the pellet for 5 min at 50°C.
6. Add 60ul of 1X loading buffer and DTT (final concentration of 0.1M).
7. Mix and boil before loading on a 15% SDS-gel and transfer onto nitrocellulose membrane. The mature Caspase-1 is detected using the Caspase-1 (p20) (human), mAb (Bally-1) antibody (Cat No 044627).
Storage and StabilityShort-term Storage: +4°C
Long-term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.
CAS Number1198-77-2
Molecular FormulaC5H3N4O3 . Na
Molecular Weight167.1+23.0
Concentration20mg/ml
FormSupplied as a liquid in PBS.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesMSU (ready-to-use), Uric acid (ready-to-use)


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