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045162 Ponasterone A (Pon A, 25-Deoxy-20-hydroxyecdysone, 25-Deoxyecdysterone, AI3-44686, 2beta,3beta,14alpha,20R,22R-Pentahydroxy-5beta-cholest-7-en-6-one) CAS: 13408-56-5

Specifications
References
CAS Number
13408-56-5
Grade
Highly Purified
Molecular Formula
C27H44O6
Molecular Weight
464.6
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C

Ponasterone A is a member of the ecdysteroid family. Ecdysone receptor (EcR) agonist. An analog of ecdysone with similar properties to muristerone A. Induces expression of β-galactosidase to levels similar to those obtained with muristerone A induction. Potent compound for ecdysteroid activity. Insect steroid hormone involved in regulating metamorphosis.

Synonyms
Pon A; 25-Deoxy-20-hydroxyecdysone; 25-Deoxyecdysterone; AI3-44686; 2β,3β,14α,20R,22R-Pentahydroxy-5β-cholest-7-en-6-one
CAS Number
13408-56-5
Solubility
Acetic acid, ethanol, methanol or DMSO. Insoluble in water. To prepare a 1mM stock solution (1.1mM) in 100% ethanol: Add 2ml of 100% ethanol to 1mg (2umol). Gently mix to dissolve the product (5 to 10min.). Do not heat solution to dissolve the product. Store the 1mM stock solution at 4°C or at -20°C.
Molecular Formula
C27H44O6
Molecular Weight
464.6
Form
Supplied as a white to off-white powder.
Purity
≥95% (HPLC)
Identity
Determined by 1H- and 13C-NMR.
Storage and Stability
Store long term at -20°C, store short term at 4°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Purity
≥95% (HPLC)
Form
Supplied as a white to off-white powder.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
Product Reference: |●Extraction of ponasterone A and ecdysterone from podocarpaceae and related plants: S. Imai et al.; Steroids 10, 557 (1967)|●Ecdysones and analogs: effects on development and reproduction of insects: W.E. Robbins, et al.; Science 161, 1158 (1968)|●The uptake and release of ponasterone A by the Kc cell line of Drosophila melanogaster: C. Beckers, et al.; Mol. Cell. Endocrinol. 17, 51 (1980)|●Past and present studies with ponasterones, the first insect molting hormones from plants: K. Nakanishi; Steroids 57, 649 (1992)|●Identification of ligands and coligands for the ecdysone-regulated gene switch: E. Saez, et al.; PNAS 97, 14512 (2000)|●Mammalian 5'(3')-deoxyribonucleotidase, cDNA cloning, and overexpression of the enzyme in Escherichia coli and mammalian cells: C. Rampazzo, et al.; J. Biol. Chem. 275, 5409 (2000)|●Sustained mammary gland-directed, ponasterone A-inducible expression in transgenic mice: C. Albanese, et al.; FASEB J. 14, 877 (2000)|●The ecdysone inducible gene expression system: unexpected effects of muristerone A and ponasterone A on cytokine signaling in mammalian cells: S. Constantino, et al.; Eur. Cytokine Netw. 12, 365 (2001)|●Recent advances in inducible expression in transgenic mice: C. Albanese, et al.; Semin. Cell. Dev. Biol. 13, 129 (2002)|●Potent and selective partial ecdysone agonist activity of chromafenozide in Sf9 cells: T. Toya, et al.; BBRC 292, 1087 (2002)|●Development of a ponasterone A-inducible gene expression system for application in cultured skeletal muscle cells: Y.Y. Xiao, et al.; Int. J. Biochem. Cell. Biol. 35, 79 (2003)|●Development of an in vitro Reporter Gene Assay for Screening the Ecdysone Receptor Agonists in Mysid Crustacean: M. Hirano, et al.; Terrapub 141 (2010)|●|
USBio References
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