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You are here:Home » Molecular Biology » MB-Enzymes, Dehydrogenase » ADH1A, Recombinant, Human, aa1-375, His-Tag (Alcohol Dehydrogenase 1A, Alcohol Dehydrogenase Subunit alpha, ADH1)

ADH1A, Recombinant, Human, aa1-375, His-Tag (Alcohol Dehydrogenase 1A, Alcohol
Dehydrogenase Subunit alpha, ADH1)

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Specifications

Alcohol dehydrogenase 1A, also known as ADH1A, belongs to the alcohol dehydrogenase family. ADH1 is a monomorphic and predominant in fetal and infant livers, becoming less active in gestation and only weakly active during adulthood. ADH1A plays a major role in ethanol metabolism. With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohols to ketones or aldehydes. The physiologic function for ADH1A in the liver is the removal of ethanol formed by microorganisms in the intestinal tract.
Catalog #045402
SourceRecombinant corresponding to aa1-375 from human ADH1A, fused to His-tag at N-terminus, expressed in E.coli.
Molecular Weight~42kD (395aa) confirmed by MALDI-TOF
AA SequenceMGSSHHHHHH SSGLVPRGSH MSTAGKVIKC KAAVLWELKK PFSIEEVEVA PPKAHEVRIK MVAVGICGTD DHVVSGTMVT PLPVILGHEA AGIVESVGEG VTTVKPGDKV IPLAIPQCGK CRICKNPESN YCLKNDVSNP QGTLQDGTSR FTCRRKPIHH FLGISTFSQY TVVDENAVAK IDAASPLEKV CLIGCGFSTG YGSAVNVAKV TPGSTCAVFG LGGVGLSAIM GCKAAGAARI IAVDINKDKF AKAKELGATE CINPQDYKKP IQEVLKEMTD GGVDFSFEVI GRLDTMMASL LCCHEACGTS VIVGVPPDSQ NLSMNPMLLL TGRTWKGAIL GGFKSKECVP KLVADFMAKK FSLDALITHV LPFEKINEGF DLLHSGKSIR TILMF
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~42kD
SourceE.coli
Purity~90% (SDS-PAGE)
Concentration~1mg/ml
FormSupplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 0.1M sodium chloride, 10% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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