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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » DUSP18, Recombinant, Human, aa1-188, His-Tag (Dual Specificity Protein Phosphatase 18, Low Molecular Weight Dual Specificity Phosphatase 20, LMWDSP20)

DUSP18, Recombinant, Human, aa1-188, His-Tag (Dual Specificity Protein Phosphatase 18, Low
Molecular Weight Dual Specificity Phosphatase 20, LMWDSP20)

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Specifications

Dual specificity phosphatase 18, also known as DUSP18, is a member of the dual-specificity phosphatase (DSP) family, which catalyzes dephosphorylation of phosphotyrosine and phosphothreonine residues. DUSP18 is inhibited by iodoarectic acid and is activated by manganese ions. Along with having preferential enzymatic activity against phosphorylated tyrosine residues over threonine residues, DUSP18 also dephosphorylates p-nitrophenyl phosphate (pNPP) in vitro. This protein is widely expressed with highest levels in liver, brain, ovary and testis.
Catalog #045422
SourceRecombinant corresponding to aa1-188 from human DUSP18, fused to His-tag at N-terminus, expressed in E.coli.
Molecular Weight~23.6kD (212aa) confirmed by MALDI-TOF
AA SequenceMGSSHHHHHH SSGLVPRGSH MGSHMTAPSC AFPVQFRQPS VSGLSQITKS LYISNGVAAN NKLMLSSNQI TMVINVSVEV VNTLYEDIQY MQVPVADSPN SRLCDFFDPI ADHIHSVEMK QGRTLLHCAA GVSRSAALCL AYLMKYHAMS LLDAHTWTKS CRPIIRPNSG FWEQLIHYEF QLFGKNTVHM VSSPVGMIPD IYEKEVRLMI PL
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~23.6kD
SourceE.coli
Purity~95% (SDS-PAGE)
Concentration~0.5mg/ml
FormSupplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 0.1mM PMSF, 0.1mM EDTA, 40% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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