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045458 RPE, Recombinant, Human, aa1-228, His-Tag (Ribulose-phosphate 3-epimerase, HUSSY-17, Ribulose-5-phosphate-3-epimerase) CAS:

Specifications
References
Grade
Highly Purified
Molecular Weight
27.5
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

RPE (ribulose-phosphate 3-epimerase) belongs to the ribulose-phosphate 3-epimerase family. In enzymology, a ribulose-phosphate 3-epimerase is the enzyme that converts D-ribulose 5-phosphate into D-xylulose 5-phosphate in Calvin's reductive pentose phosphate cycle. Hence, this enzyme has one substrate, D-ribulose 5-phosphate, and one product, D-xylulose 5-phosphate. This enzyme participates in 3 metabolic pathways: pentose phosphate pathway, pentose and glucuronate interconversions, and carbon fixation.

Source
Recombinant corresponding to aa1-228 from human RPE, fused to His-tag at N-terminus, expressed in E.coli.
Molecular Weight
~27.5kD (252aa) confirmed by MALDI-TOF
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMASGCK IGPSILNSDL ANLGAECLRM LDSGADYLHL DVMDGHFVPN ITFGHPVVES LRKQLGQDPF FDMHMMVSKP EQWVKPMAVA GANQYTFHLE ATENPGALIK DIRENGMKVG LAIKPGTSVE YLAPWANQID MALVMTVEPG FGGQKFMEDM MPKVHWLRTQ FPSLDIEVDG GVGPDTVHKC AEAGANMIVS GSAIMRSEDP RSVINLLRNV CSEAAQKRSL DR
Enzyme Activity
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥95% (SDS-PAGE)
Concentration
~0.5mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 2mM DTT, 200mM sodium chloride, 30% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Liang W., et al. (2011) FASEB J. 25:497-504. 2. Akana, J., et al. (2006) Biochemistry 45: 2493-2503.
USBio References
No references available
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