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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, Smooth Muscle (alpha-Actin 2, ACTA2)

Anti -Actin, Smooth Muscle (alpha-Actin 2, ACTA2)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant IH IC FC
Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). It has also been shown that actin dysfunction leads to certain types of heart failure (4). Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Furthermore, expression of smooth muscle alpha actin is regulated by hormones and cell proliferation, and is altered by pathological conditions including oncogenic transformation and atherosclerosis (5).
Catalog #045613
ApplicationsSuitable for use in Immunocytochemistry, Flow cytometry and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: 1:1000-1:2500
Immunocytochemistry: 1:250-1:500
Flow cytometry: 1:10-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
Clone NoEPR5368
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.01% sodium azide, 50% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues on the N-terminus in human Alpha-Actin (Smooth Muscle).
SpecificityRecognizes human Alpha-Actin (Smooth Muscle). Species crossreactivity: Mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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