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You are here:Home » Antibodies » Abs to Nucleotides » Anti -5-Hydroxymethylcytosine (5hmC, 5'OH methyl cytosine)

Anti -5-Hydroxymethylcytosine (5hmC, 5'OH methyl cytosine)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified IC
5-Hydroxymethylcytosine in the mammalian zygote is linked with epigenetic reprogramming.
5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base formed from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of iron-dependent oxygenases. Data suggests that every mammilian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; data also suggests that levels of 5-hydroxymethylcytosine increases with age. The highest levels are found in neuronal cells of the central nervous system and certain mammalian tissues such as mouse Purkinje and granule neurons. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethlcytosine may regulate gene expression or initiate DNA demethylation.
Catalog #045666
ApplicationsSuitable for use in Dot Blot
Recommended DilutionDot Blot: 10ug/ml and 1ug/ml detected 5-hydroxymethylcytosine in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA.
ControlUnmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone NoAB3/63.3
HostRat
Concentration0.5mg/ml
FormSupplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM NaCl, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
Immunogen5-Hydroxymethylcytosine conjugated to BSA
SpecificityDetects 5-hydroxymethyl cytosine, but not 5-methylcytosine or unmethylated cytosine. Non-species specific antibody against modified DNA. Expected to detect 5-hydroxymethylcytosine in all species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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