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You are here:Home » Antibodies » Abs to Neuroscience » Anti -AMPH (Amphiphysin, AMPH1)

Anti -AMPH (Amphiphysin, AMPH1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B
AMPH is a member of the Amphiphysin family, BAR superfamily of proteins. Although its calculated MW is only 78kD, it runs anomalously in SDS-PAGE at 125-128kD. It is expressed in neurons, Sertoli cells, possibly in anterior pituitary endocrine cells and apparently cardiac muscle. It may form heterodimers with AMPH2, and in this configuration, promotes clathrin-associated endocytic vesicle formation. Furthermore, and perhaps related, AMPH also interacts with N-WASP, initiating actin polymerization. Human AMPH is 695aa in length. It contains an N-terminal BAR domain aa24-240 that binds lipids, a central region that binds AP2 aa322-340 and clathrin aa347-386, and a C-terminal SH3 domain aa622-695 that binds proline-rich ligands. There is one known 108kD splice variant that shows a deletion of aa425-466. Over aa204-300, human AMPH shares 98% aa identity with mouse AMPH.
Catalog #123296
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
AA SequenceMADIKTGIFAKNVQKRLNRAQEKVLQKLGKADETKDEQFEEYVQNFKRQEAEGTRLQRELRGYLAAIKGMQEASMKLTESLHEVYEPDWYGREDVKMVGEKCDVLWEDFHQKLVDGSLLTLDTYLGQFPDIKNRIAKRSRKLVDYDSARHHLEALQSSKRKDESRISKAEEEFQKAQKVFEEFNVDLQEELPSLWSRRVGFYVNTFKNVSSLEAKFHKEIAVLCHKLYEVMTKLGDQHADKAFTIQGAPSDSGPLRIAKTPSPPEEPSPLPSPTASPNHTLAPASPAPARPRSPSQTRKGPPVPPLPKVTPTKELQQENIISFFEDNFVPEISVTTPSQNEVPEVKKEETLLDLDFDPFKPEVTPAGSAGVTHSPMSQTLPWDLWTTSTDLVQPASGGSFNGFTQPQDTSLFTMQTDQSMICNLAESEQAPPTEPKAEEPLAAVTPAVGLDLGMDTRAEEPVEEAVIIPGADADAAVGTLVSAAEGAPGEEAEAETATVPAGEGVSLEEAKIGTETTEGAESAQPEAEELEATVPQEKVIPSVVIEPASNHEEEGENEITIGAEPKETTEDAAPPGPTSETPELATEQKPIQDPQPTPSAPAMGAADQLASAREASQELPPGFLYKVETLHDFEAANSDELTLQRGDVVLVVPSDSEADQDAGWLVGVKESDWLQYRDLATYKGLFPENFTRRLD
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgM,k
Clone No1H3-1D11
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in ascites fluid.
PurityAscites
ImmunogenFull length recombinant corresponding to aa1-695 from human AMPH (AAH34376) with GST tag. MW of the GST tag alone is 26kD.
SpecificityRecognizes human AMPH.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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