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123464 Mouse Anti-ARF5 (ADP-ribosylation Factor 5)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P84085
Isotype
IgG1,k
Clone Number
1B4
Grade
Affinity Purified
Applications
E IF WB
Crossreactivity
Hu Mo Rt
Accession #
BC003043, AAH03043
Shipping Temp
Blue Ice
Storage Temp
-20°C

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

Applications
Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Immunofluorescence: 15ug/ml Optimal dilutions to be determined by the researcher.
AA Sequence
YFQNTQGLIFVVDSNDRERVQESADELQKMLQEDELRDAVLLVFANKQDMPNAMPVSELTDKLGLQHLRSRTWYVQATCATQGTGLYDGLDWLSHELSKR
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Partial recombinant corresponding to aa81-180 from human ARF5 (AAH03043) with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human ARF5. Species Crossreactivity: mouse and rat.
References
1. GBF1-Arf-COPI-ArfGAP-mediated Golgi-to-ER transport involved in regulation of lipid homeostasis. Takashima K, Saitoh A, Hirose S, Nakai W, Kondo Y, Takasu Y, Kakeya H, Shin HW, Nakayama K.Cell Struct Funct. 2011;36(2):223-35. 2. Class II ADP-Ribosylation factors are required for efficient secretion of dengue viruses. Kudelko M, Brault JB, Kwok K, Li MY, Pardigon N, Peiris JS, Bruzzone R, Despres P, Nal B, Wang PG.J Biol Chem. 2011 Nov 21. 3. Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity. Puxeddu E, Uhart M, Li CC, Ahmad F, Pacheco-Rodriguez G, Manganiello VC, Moss J, Vaughan M.Proc Natl Acad Sci U S A. 2009 Apr 14;106(15):6158-63. Epub 2009 Mar 30. 4. EFA6 facilitates the assembly of the tight junction by coordinating an Arf6-dependent and independent pathway. Klein S, Partisani M, Franco M, Luton F.J Biol Chem. 2008 Oct 31;283(44):30129-38. Epub 2008 Sep 8. 5. The brefeldin A-inhibited guanine nucleotide-exchange protein, BIG2, regulates the constitutive release of TNFR1 exosome-like vesicles. Islam A, Shen X, Hiroi T, Moss J, Vaughan M, Levine SJ.J Biol Chem. 2007 Mar 30;282(13):9591-9. Epub 2007 Feb 2.
USBio References
No references available
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