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You are here:Home » Antibodies » Abs to Apoptosis, Caspase » Anti -CARD8 (Caspase Recruitment Domain-containing Protein 8, Apoptotic Protein NDPP1, CARD-inhibitor of NF-kappa-B-activating Ligand, CARDINAL, DACAR, DAKAR, DKFZp779L0366, FLJ18119, FLJ18121, KIAA0955, MGC57162, NDPP1, NDPP, Tumor Up-regulated CARD-containing

Anti -CARD8 (Caspase Recruitment Domain-containing Protein 8, Apoptotic Protein NDPP1,
CARD-inhibitor of NF-kappa-B-activating Ligand, CARDINAL, DACAR, DAKAR, DKFZp779L0366,
FLJ18119, FLJ18121, KIAA0955, MGC57162, NDPP1, NDPP, Tumor Up-regulated CARD-containing

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Mouse Affinity Purified B IF
TUCAN (also known as CARD8 and CARDINAL) is a CARD domain containing protein. TUCAN stands for tumor-up-regulated CARD-containing antagonist of caspase nine. Proteins containing a CARD (caspase-associated recruitment domain) domain are key regulators of cell death, cell survival and cytokine production (reviewed in Dmiano and Reed, 2004). CARD domains are found in the N-terminal pro-domains of certain caspases, a family of apoptotic and pro-inflammatory proteases, as well as in a diversity of other proteins including TUCAN. CARD domains are homotypic protein interaction motifs that enable networks of proteins to communicate via CARD-CARD interactions. TUCAN is an anti-apoptotic CARD protein that can protect tumors from cell death stimuli, and is overexpressed in certain forms of cancer. The role of TUCAN in tumor biology remains to be fully elucidated, and there appears to be be various mechanisms by TUCAN can potentially contribute to apoptosis resistance (reviewed in Checinska et al, 2006). For example, TUCAN has been shown to inhibit caspase-9 activation by binding to the CARD region of pro-caspase-9, thereby suppressing the formation of the Apaf-1-caspase-9 apoptotic complex and apoptosis. Additionally, a TUCAN isoform has been described that blocks both caspase-8 and caspase-9 mediated apoptosis. However, in some tumors, TUCAN does not appear to interact with caspase-9 and may play a role in modulating NF-kB transcription factor survivial signaling pathways. In this regard a CARD-independent interaction of TUCAN with Ikkg has been described, resulting in the inhibition of interleukin-1 and TNF-induced NF-kB activation.
Catalog #124342
ApplicationsSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionImmunofluorescence: 20ug/ml
Optimal dilutions to be determined by the researcher.
AA SequenceMEKKECPEKSSSSEEELPRRDSGSSRNIDASKLIRLQGSRKLLVDNSIRELQYTKTGIFFQAEACVTNDTVYRELPCVSETLCDISHFFQEDDETEAEPLLFRAVPECQLSGGDIPSVSEEQESSEGQDSGDICSEENQIVSSYASKVCFEIEEDYKNRQFLGPEGNVDVELIDKSTNRYSVWFPTAGWYLWSATGLGFLVRDEVTVTIAFGSWSQHLALDLQHHEQWLVGGPLFDVTAEPEEAVAEIHLPHSISLQAGEVDVSWFLVAHFKNEGMVLEHPARVEPFYAVLESPSFSLMGILLRIASGTRLSIPITSNTLIYYHPHPEDIKFHLYLVPSDALLTKAIDDEEDRFHGVRLQTSPPMEPLNFGSSYIVSNSANLKVMPKWISSL
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2.
PurityPurified by Protein A affinity chromatography.
ImmunogenFull length human CARD8, aa1-392 (AAH56891.1).
SpecificityRecognizes human CARD8.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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