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You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -GLI3 (Transcriptional Activator GLI3, GLI3 Form of 190kD, GLI3-190, GLI3 Full Length Protein, GLI3FL)

Anti -GLI3 (Transcriptional Activator GLI3, GLI3 Form of 190kD, GLI3-190, GLI3 Full Length
Protein, GLI3FL)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IF
Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.
Catalog #127344
ApplicationsSuitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended DilutionImmunofluorescence: 10ug/ml
Optimal dilutions to be determined by the researcher.
AA SequenceMEAQSHSSTTTEKKKVENSIVKCSTRTDVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPQNVQGLSKVSEEPSTSSDERASLIKKEIHGSLPHVAEPSVPYRGTVFA
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No2C9
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2.
PurityPurified by Protein A affinity chromatography.
ImmunogenPartial recombinant corresponding to aa1-110 from human GLI3 (NP_000159) with GST tag. MW of the GST tag alone is 26kD.
SpecificityRecognizes human GLI3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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